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AEI currently utilizes a RIEGL VZ400 Terrestrial Laser Scanner on various job sites.  This proecss utilizes a high accuracy 3D terrestrial LiDAR unit.  The data collected will be brought into a feature extraction software, TopoDOT.  TopoDOT utilizes a variety of tools to identify adn quickly extrapolate features within pointcloud data.  All features can then be imported into an AutoCAD drawing file.  AEI can provide an accurate 3D pointcloud of terrain plus any existing building or structures.  The pointcloud can also be converted for Building Information Models.

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Allen Engineering is involved with the civil design and surveying for the new park in Palm Bay, Flordia.  This Regional Park will feature 150 full service campsite hookups and is scheduled to break ground in 2018.  We are extremely proud to be involved in this project.

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Allen Engineering is beginning its 21st year associated with the Space Coast Post of the Society of American Military Engineers (SAME).  During our 21 years, we have helped raise over $350,000 in scholarships and endowments.  We are extremely proud to be associated with SAME and its continued commitment to offer opportunities for students pursuing careers in the engineering field.


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  • Cooperating Associate Professor of Sports Medicine, University of Maine
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Process each specimen as appropriate for that specimen and inoculate directly onto the surface of the medium blood pressure and anxiety cardizem 120mg free shipping. Count mold colonies from duplicate plates and record average count as mold count per gram or milliliter of sample arrhythmia practice test purchase cardizem 60mg on-line. The formulation was evaluated by many investigators who reported it as a suitable alternative medium for standard plate counts blood pressure chart time of day cardizem 60mg without a prescription. Water Sample Principles of the Procedure Peptone provides nitrogen and carbon as well as other nutrients blood pressure chart teenager order cardizem 180mg with visa. Place dishes in a close-fitting box or plastic bag containing moistened paper towels. Expected Results Count all colonies on the membrane when there are 2 or less colonies per square. For 3-10 colonies per square, count 10 squares and obtain average count per square. For 10-20 colonies per square, count 5 squares and obtain average count per square. Multiply average count per square by 100 and divide by the sample volume to give colonies per milliliter. If there are more than 20 colonies per square, record count as > 2,000 divided by the sample volume. Haemophilus Isolation Agar with Bacitracin Intended Use Haemophilus Isolation Agar with Bacitracin is a primary plating medium used for the selective isolation of Haemophilus species. IsoVitaleX Enrichment is a chemically-defined supplement that provides V factor and other nutrients, such as thiamine and cysteine, to stimulate the growth of Haemophilus species. The polypeptide antibiotic bacitracin is incorporated into these media to inhibit normal flora, including gram-positive bacteria, such as streptococci, and most species of Neisseria. Horse blood provides appropriate hemolytic reactions to facilitate the differentiation of Haemophilus species. The antimicrobial agent bacitracin is incorporated to inhibit the growth of bacteria that could mask the presence of Haemophilus species. Bacitracin is frequently utilized in enriched media as a selective agent to increase the recovery of Haemophilus species from the upper respiratory tract. Principles of the Procedure Haemophilus Isolation Agar with Bacitracin consists of Brain Heart Infusion Agar supplemented with Fildes Enrichment, IsoVitaleX Enrichment and horse blood. Fildes Enrichment is a peptic digest of sheep blood that supplies both X and V factors. Summary and Explanation Principles of the Procedure the Bauer-Kirby procedure is based on the diffusion through an agar gel of antimicrobial substances which are impregnated on paper discs. Paper discs impregnated with specified amounts of antibiotic or other antimicrobial agents are then placed on the surface of the medium, the plate is incubated and zones of the inhibition around each disc are measured. These include the medium, agar depth, disc potency, inoculum concentration, pH and beta-lactamase production by test organisms. Prepare a Gram stain before starting susceptibility testing to confirm culture purity and to confirm tentative identification of Haemophilus. Use several well-isolated colonies taken directly from an overnight (preferably 20-24 hours) Chocolate Agar plate as the source of the inoculum. A rapid -lactamase test should be utilized for rapid detection of strains that are resistant to penicillin, ampicillin or amoxicillin. Care must be exercised in preparing this suspension because higher inoculum concentrations may lead to false-resistant results with some -lactam antibiotics, particularly when -lactamase-producing strains of H. Disregard faint growth of tiny colonies which can be detected with difficulty near the edge of the obvious zone of the inhibition. Cumitech 6A, New developments in antimicrobial agent susceptibility testing, A practical guide. If only isolated colonies grow, the inoculum was too light and the rest should be repeated. Measure the diameter of the zones of complete inhibition (as judged by the unaided eye), including the diameter of the disc, to the nearest whole millimeter, using calipers, a ruler, or a template prepared for this purpose. Heart Infusion Agar Intended Use Heart Infusion Agar is a general-purpose medium used in the cultivation of a wide range of microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Heart Infusion Agar derives its nutrients from heart muscle infusion and peptone, which supply nitrogenous and carbonaceous compounds, sulfur, vitamins and trace ingredients. The addition of 5% sheep blood provides additional growth factors and is used to determine hemolytic reactions. Summary and Explanation Meat infusions provided one of the earliest means of culturing microorganisms, and infusion-based media are still widely used. Huntoon demonstrated that pathogenic organisms could be grown on infusion agar without supplements. Heart infusion media are specified for the isolation of Vibrio cholerae and Vibrio species. Using a sterile inoculating loop or needle, pick several isolated colonies from the primary isolation plate and streak the surface of a slant of Heart Infusion Agar. One of the first media used for the cultivation of bacteria was a liquid medium containing an infusion of meat. Huntoon1 using fresh beef heart and Bacto Peptone, prepared a "hormone" broth to retain growth promoting substances.

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Ironically yaz arrhythmia generic 120 mg cardizem fast delivery, the many medical challenges and ultimate loss of others in this close network can be threatening to hypertension journals order cardizem 120 mg overnight delivery other families and can add to hypertension 5 mg purchase cardizem 60mg on line the cumulative losses experienced by this unique group blood pressure medicine side effects order cardizem 180mg with visa. It is an emotional, physical, and spiritual necessity, the price you pay for love. In my experience, anti-depressants and even therapy did not help (although both can help many). I finally concluded that I owned this grief, and if life could ever become more bearable, I had to walk right through the middle of the most painful feelings imaginable. I had to cry (incessantly, my husband would say), and I had to express my deepest feelings if a special friend would listen. I also had to find those caring family members, friends, and physical activities that would bring positive energy to my life. I had to walk, immerse myself in the beauty of nature, ski down a mountain, and listen to the classical music that brought peace and joy into my life. Some find comfort in creative and artistic pursuits, in journaling, in prayer, and in meditation or mindfulness. Reaching out to people in need or devoting energy to a cause that serves others can be therapeutic. Many parents affirm that their religious beliefs have been crucial to their emotional survival. And yet some get "stuck" in the grieving process and find it extremely difficult to function over an extended period of time. In these situations, professional help may be essential to move through the most painful phases of grieving. Elizabeth Kubler-Ross and John Kessler Parents frequently believe that they will never experience happiness again and that the depression and deep sadness they feel will be with them forever. Over a period of months and even years, the pain one initially experiences will lessen. Parents find increased energy to engage with other people, to work with energy and purpose, and to follow new pursuits. But I believe that when a parent loses a child, the parent never really "gets over" the loss. The special relationship you cherished, the precious time you shared, and the essence of the one you lost is forever a part of you. And in spite of your suffering, you might well admit that you were truly fortunate to be the parent of this wonderful human being. Many acknowledge that, in spite of what they and their child have been through, they would forever choose the time they had, than never to have known this child at all. The hole in my heart shall always remain, but there is healing around that hole, and I carry my daughter in my heart, always. The physician can play a crucial role in helping the family move from the depths of despair, anger, and self-blame into understanding the disease, making and participating in a treatment plan, and maintaining hope. The treating physician needs to be willing to learn, eager to explore current literature and to seek out information from experts. The doctor must be willing to invest the time to learn new therapeutic approaches. It is extremely helpful if the physician is a caring, warm individual, concerned about the welfare of the patient and aware of the stress the family is experiencing. Physicians need to listen to fears and concerns, and answer questions in understandable terms. It is crucial that they give families the time they need to ask questions, and listen to their concerns and feelings. Physicians may be helpful in encouraging the family to ask difficult questions that fear may cause family members to avoid. Richard Sills, sat down with us very late one night explaining, reviewing, and answering every single one of our questions and fears. Statistics do not include the high probability that bone marrow transplant outcomes will continue to improve, that new methods of gene therapy could change life expectancies, and that future discoveries will improve overall survival rates. Families need to know that scientific discoveries concerning this rare disorder have progressed at a very rapid pace over more than a decade and that many laboratories are actively pursuing new and hopeful approaches. They can unwittingly create an atmosphere of sadness and worry which permeates every day and which children immediately sense. As a result, the time that is shared between parent and child may not be "quality time" at all. Entering into a partnership with families Family members should be encouraged to play an active role in the treatment plan. Making families part of the decision-making process enables them to cope with the anxiety, depression, and loss of control they are experiencing. The relationship between physician and family should be one of mutual respect, shared information, and joint decision-making. The doctor should encourage family members to voice their concerns or disagreements with the treatment plan. Parents and patients are often intimidated by medical authority, or fear appearing foolish by asking inappropriate questions. But they must live with the results of any medical intervention, so they must understand and agree with decisions.

Procedure Refer to pulse pressure pda buy 120 mg cardizem with visa appropriate references for specific procedures on the isolation and cultivation of fungi enrique iglesias heart attack generic cardizem 120mg otc. Cooked Meat Medium with Glucose blood pressure and age cardizem 180mg on-line, Hemin and Vitamin K1 blood pressure medication karvezide 120mg cardizem visa, is also recommended as a subculture medium for anaerobic isolates to be examined by gas liquid chromatography. Cooked Meat Medium is still widely used for the cultivation and maintenance of clostridia and for determining proteolytic activity of anaerobes. For example, the medium is recommended for use in the enumeration and identification of Clostridium perfringens from food. The medium is also useful as an enrichment broth for cultivating anaerobes that may be present in small numbers in a population and as a subculture medium for determination of proteolysis (meat digestion) and spore formation by Clostridium species. Principles of the Procedure Cooked Meat Medium provides a favorable environment for the growth of anaerobes, since the muscle protein in the heart tissue granules is a source of amino acids and other nutrients. The muscle tissue also provides reducing substances, particularly glutathione, which permits the growth of strict anaerobes. Cooked Meat Medium with Glucose, Hemin and Vitamin K1 is supplemented with added glucose, yeast extract, hemin and vitamin K1 to enhance the growth of anaerobic microorganisms. Growth is indicated by turbidity and, with some organisms, by the presence of gas bubbles in the medium. Solution is medium amber, clear to very slightly opalescent supernatant fluid over insoluble pellets. Medium amber, clear to very slightly opalescent supernatant fluid over insoluble pellets. Using a sterile inoculating loop or needle, transfer growth from a fresh subculture medium, inoculating heavily in the area of meat particles. Growth of proteolytic organisms is generally characterized by blackening and dissolution of the meat particles. Organisms to be cultivated must first be isolated in pure culture in an appropriate medium. With the addition of polysorbate 80, it is utilized primarily for the testing of Candida species for their ability to produce chlamydospores. Pollack and Benham reported on its usefulness for studying Principles of the Procedure Corn Meal Agar is a relatively simple medium, consisting of an infusion of corn meal and agar. The infusion product contains sufficient nutrients to support the growth of fungal species. The polysorbate 80 is a mixture of oleic esters which, when added to the basal medium, stimulates the production of chlamydospores. Make an S-shape by lightly streaking back and forth across the two parallel streak lines. Procedure To prepare plated media from agar deeps, place the agar deeps in a boiling water bath until the medium becomes liquefied (clear). Pour the molten medium into a sterile Petri dish and allow to solidify before use. Organisms to be cultivated for identification must first be isolated in pure culture on an appropriate medium. Corn Meal Agar with 1% Dextrose should be incubated for up to 4 weeks to allow sufficient time for pigmentation to develop. Test for the production of chlamydospores on medium containing polysorbate 80 using the Dalmau plate method. Then lightly make an S-shaped streak back and forth across the two original streak lines. Flame a coverslip and, after it cools, place it over the central area of the stab marks to provide slightly reduced oxygen tension. If the test is negative, reincubate plates an additional 48-72 hours and examine again. Cystine Assay Medium (See Amino Acid Assay Media) Cystine Heart Agar Intended Use Cystine Heart Agar is used with hemoglobin for cultivating Francisella tularensis and without enrichment for cultivating gram-negative cocci and other microorganisms. Cystine Heart Agar, also known as Cystine Glucose Blood agar, is the historical medium of choice for isolating F. Principles of the Procedure Infusions from beef heart, peptone and L-cystine provide nitrogen, vitamins and amino acids in Cystine Heart Agar. Without enrichment, Cystine Heart Agar supports excellent growth of gram-negative cocci and other pathogenic microorganisms. Solution is light to medium amber, very slightly to slightly opalescent, may have fine precipitate. For a complete discussion on the inoculation and identification of Francisella, consult appropriate references. Overgrowth by contaminating organisms can be reduced by incorporating 100-500 units penicillin per mL into the medium. Czapek-Dox media are useful in a variety of microbiological procedures, including soil microbiology and fungi and mildew resistance tests. Thom and Raper3 reported Czapek-Dox Broth and Czapek Solution Agar produce moderately vigorous growth of most saprophytic aspergilli and yield characteristic mycelia and conidia. Czapek Solution Agar is recommended in Standard Methods for the Examination of Water and Wastewater5 for the isolation of Aspergillus, Penicillium, Paecilomyces and related fungi. Dipotassium phosphate is the buffering agent, and potassium chloride contains essential ions.

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Kimura K prehypertension exercise order cardizem 180mg online, Katsumata Y blood pressure 300180 cardizem 120mg amex, Ozawa T blood pressure medication can you get off purchase 180 mg cardizem mastercard, Tawara S blood pressure medication headache purchase cardizem 60 mg overnight delivery, Igarashi K, Cho Y, Shibata N, Hakuno F, Takahashi S-I, Takenaka A. Effect of paraquat-induced oxidative stress on insulin regulation of insulin-like growth factor-binding protein-1 gene expression. Synergistic effects of low level stressors in an oxidative damage model of spinal motor neuron degeneration. Responses of planktonic microorganisms from tropical reservoirs to paraquat and deltamethrin exposure. Etiology and outcome of patients presenting for poisoning to the emergency department in Taiwan: a prospective study. Chemically diverse toxicants converge on Fyn and c-Cbl to disrupt precursor cell function. Effects of paraquat on the substantia nigra of the Wistar rats: neurological, histological, and behavioral studies. Environmental risk factors and Parkinson`s disease: a case control study in Taiwan. Cyclooxygenase-2 de ciency modi es the neurochemical effects, motor impairment and co-morbid anxiety provoked by paraquat administration in mice. The herbicide paraquat causes up-regulation and aggregation of a-synuclein in mice. Rotterdam Convention on the Prior Informed Consent Procedure for Certain Hazardous Chemicals and Pesticides in International Trade, Chemical Review Committee, Fifth meeting, Rome, 2327 March, 2009. Evidence for transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state. The effect of free radicals induced by paraquat and copper on the in vitro development of Plasmodium falciparum. Environmental risk factors and Parkinson`s disease: selective degeneration of nigral dopaminergic neurons caused by the herbicide paraquat. Effects of prenatal paraquat and mancozeb exposure on amino acid synaptic transmission in developing mouse cerebellar cortex. Alter-native electron acceptors: Proposed mechanism of paraquat mitochondrial toxicity. Pesticide risk assessment: a study on inhalation and dermal exposure of 2,4-D and paraquat among pesticide operators. Mollace V, Iannone M, Muscoli C, Palma E, Granato T, Rispoli V, Nistico R, Rotiroti D, Salvemini D. The role of oxidative stress in paraquatinduced neurotoxicity in rats: protection by non peptidyl superoxide dismutase mimetic. Parental occupational exposure to pesticides and the risk of childhood leukaemia in Costa Rica. Pollution problems in the South Paci c: fertilizers, biocides, water supplies and urban wastes. Intentional poisoning of animals in southeastern Spain: a review of the veterinary toxicology service from Murcia, Spain. Surveillance of pesticide-related illness in the developing world: Putting the data to work. Paraquat-induced oxi-dative stress response during amphibian early embryonic development. Nagami H, Nishigaki Y, Matsushima S, Matsushita T, Asanuma S, Yajima N, Usuda M, Hirosawa M. Paraquatgenerated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase. Toxicity of Gramoxone (paraquat) to Juvenile African Cat sh, Ciarias gariepinus (Burchell, 1822). Teratogenic effects of amitraz, 2,4-dimethylaniline, and paraquat on developing frog (Xenopus) embryos. Cancer incidence among paraquat exposed applicators in the Agricultural Health Study: a prospective cohort study. Competitive and non-competitive adsorption, desorption of paraquat, diquat and difenzoquat in vineyard-devoted soils. The herbicide paraquat induces alterations in the elemental and biochemical composition of non-target microalgal species. Microglial activation as a priming event leading to paraquat-induced 41 dopaminergic cell degeneration. Effects of paraquat and glyphosate on steroidogenesis in gonads of the frog Rana esculenta in vitro. Risk assessment as a management tool used to assess the effect of pesticide use in an irrigation system, situated in a semi-desert region. Salazar-Lugo R, Estrella A, Oliveros A, Rojas-Villarroel E, Villaobos de B L, Lemus M. Paraquat and temperature affect non-speci c immune response of Colossoma macropomum. Pulmonary function and exercise-associated changes with chronic low-level paraquat exposure. Shibata M, Hakuno F, Yamanaka D, Okajima H, Fukushima T, Hasegawa T, Ogata T, Toyoshima Y, Chida K, Kimura K, Sakoda H, Takenaka A, Asano T, Takahashi S-I. Shimizu K, Ohtaki K, Matsubara K, Aoyama K, Uezono T, Saito O, Suno M, Ogawa K, Hayase N, Kimura K, Shiono H. Carrier-mediated processes in blood-brain barrier penetration and neural uptake of paraquat.

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As reported in the section on Toxicology blood pressure medication that does not cause joint pain buy 60mg cardizem mastercard, several studies have shown paraquat to blood pressure chart 17 year olds buy cardizem 180mg free shipping be embryotoxic and teratogenic to blood pressure medication helps acne cardizem 180 mg on-line frogs heart attack left or right discount cardizem 60 mg mastercard. Maternal exposure results in higher embryo and tadpole mortality, as well as growth retardation, abnormal tail exure and gut coiling, and stunted growth rate in surviving tadpoles (Vismara et al 2000, 2001a, 2001b; Osano et al 2002), prompting Vismara et al (2000) to describe paraquat as highly embryotoxic for amphibia, and Osano et al to conclude that paraquat should be classi ed as a teratogen. Dial & Bauer (1984) also reported teratogenic effects in young developing Rana pipiens tadpoles after treatment, at paraquat concentrations as low as 0. Mussi & Calcaterra (2010) found that exposure to paraquat reduced the ability of embryos of the toad Chaunus arenarum to develop normally, leading to arrested development and severe malformations such as tail abnormalities, abdominal edema, reduced head development, and curved dorsal structures. Paraquat inhibited the production of testosterone in the testis and 17beta-oestradiol in the ovary of the frog Rana esculenta (Quassinti et al 2009). It may also be affected, directly or indirectly, by downstream movement of paraquat when streams travel through treated areas, up to 300 km for forest land cover, 258 km for cultivated crops, and 88. Paraquat adversely affects freshwater shrimps, causing reduced feeding, body weight, and oxygen consumption (Yuan et al 2004); and reducing their ability to respond to chemical attractants (Chu & Lau 1994). It has a teratogenic effect on sea squirt larvae, involving malformation of the nervous system and decreases in dopamine (Zega et al 2010). They also found sublethal effects on gene expression at exposure levels as low as 0. Plants can concentrate high levels of paraquat from water, with residues of 2,300 mg/kg and 1,300 mg/kg reported in Chara sp. Plankton In a study of freshwater reservoirs in western Africa, paraquat adversely affected aquatic microorganisms including bacteria at concentrations as low as 5. These results indicate that paraquat can cause signi cant ecological disturbances in freshwater ecosystems through alterations in species composition, potentially resulting in loss of biodiversity, harmful algal blooms, disease, and decline in sheries. The European Commission`s Scienti c Committee on Plants expressed concern in 2002 about the effects of paraquat on wildlife welfare, especially on hares and birds. Nevertheless exposure, especially chronic exposure, remains a risk and especially to reproduction. Eisler (1990) reported that some birds are very much more sensitive than others, with adverse effects at 10 mg/kg bw in nestlings of the American kestrel (Falco sparverius) causing reduced growth; 20 mg/kg in the diet of northern bobwhite (Colinus virginanus) causing reduced egg deposition; and 40 mg/L in the drinking water of domestic chickens (Gallus sp. The lowest doses of paraquat causing measurable adverse effects in sensitive species of birds were 0. Other signs appearing after 3 hours include diarrhoea, ruf ed feathers, lack of coordination, imbalance, wing drop, slowness, weakness, running and falling, constriction of pupils, and terminal convulsions. Additional signs reported after dermal exposure include blistering and cracking of skin, lacrimation, wingspread, and wing shivers. At concentrations less than the recommended application rate, paraquat is embryotoxic to developing eggs of migratory waterfowl (0. Paraquat has caused pseudofeminization of male chicken and quail embryos; testes showed intersexual phenomena and Mullerian duct abnormalities; both sexes had a reduction in the number of gonocytes (germ cells responsible for spermatogenesis in males, and oogenesis in females (Haley 1979; Eisler 1990). During one dry spell of weather paraquat spraying in a eld resulted in small puddles to which bees were attracted and subsequently died. Micro-organisms Paraquat is toxic to soil fungi and bacteria causing a reduction in some populations (Sahid et al 1992). It has also been found, in combination with diquat, to increase populations 32 of some pathogens, such as Gaeumannomyces graminis var. It was found to be toxic to 29 out of 35 strains of the nitrogen- xing bacteria Rhizobium, which play an important role in maintaining soil fertility (Martani et al 2001; Marino et al 2008). Paraquat is also toxic to the bene cial nitrogen- xing bluegreen alga Cylindrospermum sp. Symptoms of affected sheep included depression, head held low, uncoordinated gait, reluctance to move, yellow diarrhoea, and dehydration (Philbey & Morton 2001). However other authors report that paraquat can be signi cantly degraded by bacteria, fungi, actinomyces and yeast, using the paraquat as a nitrogen source (Amondham et al 2006). However Amondham et al (2006) claim that it does, and Eisler (1990) states that 50% of paraquat in the surface soils photodecomposes in 3 weeks whilst that in subsurface soils does not. In eld studies in Thailand, only 25% of the paraquat remained after 3 months; the faster degradation is attributed to higher temperatures and intensive solar radiation causing photodegradation (Amondham et al 2006). It binds readily to both clay and organic matter, with adsorption increasing with clay content. A trial using vineyard soils in Spain found 70-90% of paraquat was adsorbed, but 11% was desorbed again (Pateiro-Moure et al 2010). Adsorption increases with increasing pH, and decreases with increasing acidity (Muhamad et al 2010). For example in kaolinite clay the paraquat slowly became available to plant roots and killed cucumber seedlings, whereas in soil with 1% montmorillonite it was not available. At the same time adsorption of paraquat onto clay minerals affects their capacity for holding water and nutrients (Weber & Scott 1966). The strong adherence to soil limits the availability of paraquat to plants or other organisms; hence 33 5. Paraquat is adsorbed onto suspended matter in water, and onto sediment, with "no evidence of desorption. According to Eisler (1990), loss of paraquat from the water phase is rapid: about 50% in 36 hr and 100% in 4 weeks from freshwater ecosystems; and in marine ecosystems, 50-70% loss of paraquat from seawater was usually recorded within 24 hr. Paraquat is likely to enter surface waters bound to soil particles as a result of erosion and run-off, and subsequently be redeposited onto the beds of surface water bodies or lowland areas that receive eroded sediments from uplands. Paraquat was the most commonly found pesticide in the sediment of watershed areas for Davao City, Philippines.

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